McMaster Microscope Slide Faecal Egg Counting - Plain / Blue Grid Lines
McMaster Faecal Egg Counting Microscope Slide
McMaster counting slides are widely used for performing faecal worm egg counts. Faecal worm egg counts are performed on faeces samples mainly from large animals such as horses, sheep, cattle, and other ruminants which normally harbour low levels of worms in their guts. This allows evaluation of the efficacy of the worm drench or drenching program being used and also the likely pasture contamination in a given area. For use with 10X objective microscopes. Slide can be cleaned and reused. Simply rinse the slide thoroughly under warm running water. Instructions for use are included.Please click here to see the Manual Sheet.
Please click here to see the Fact Sheet.
Select in the Drop Down Box Above from Two Versions: the Plain Slide or the Blue Grid Slide. We highly recommend the improved Blue Grid Slide version as it makes for considerably easier recognition of the grid lines.
Modified McMaster Testing Method:
- McMaster's Method Slide
- 50ml beaker
- Pipette, Tweezers
- Microscope (Mechanical stage recommended)
- Flotation solution (16oz warm water + 3/4c. table salt)
- Fresh fecal sample for testing
1. Measure 26ml of flotation solution into beaker. Add small bits of fecal sample until beaker measures 30 ml.
2. Crush and mix the fecal sample to distribute any possible eggs.
3. While stirring beaker contents with the pipette, suction liquid.
4. Fill the McMaster chambers; place within the microscope stage.
5. Count the parasite eggs for each type of parasite in both chambers and record separately.
6. Multiply the total eggs counted per parasite species by 25. This is your Eggs Per Gram.
Fecal Egg Count Reduction Test (FECRT)
The faecal egg count reduction (FECR) in faeces is the most widely used method to assess the efficacy of anthelmintic against gastrointestinal strongyles. Administration of an anthelmintic does not guarantee the animal has been dewormed. A Fecal Egg Count Reduction Test (FECRT) can be used to assess the anthelmintic efficacy by calculating the percentage of parasites that survived treatment of the drug administered.
To perform the test:
Conduct a FEC before deworming followed by a second FEC 7, 10 or 14 days post treatment (varies with drug). A reduction of less than 90-95% indicates resistance.
Drug - Days-post Treatment:
- Pyrantel: 7
- Benzimidazoles (Fenbendazole) : 10
- Avermectins (Ivermectin, Moxidectin) : 14
EPG (pre-treatment) – EPG (14 day post-treatment) X 100 = FECRT
Pre FEC (500) – Post FEC (25) = 475
475/500 (pre) = 0.95 x 100 = 95%
Slide Care Instructions:
- Thoroughly rinse out the McMaster chambers with warm running water.
- Let slide air dry.
If the chamber becomes dirty soak for only a few minutes in warm water containing dish soap; do not soak in soap for long periods, as this will cause the chamber to become cloudy.
- Egg per gram sensitivity : 25 epg*
- Grid size : 1 cm x 1 cm
- Volume under grid : 0.15 ml
- Grid Style : Engraved
- Material : Acrylic
- Slide dimensions : 2.5 cm x 7.5 cm
- Grid Colour : Standard Opaque or Blue (select above)